Journal: Bone & Joint Research

Article Title: Tibial cortex transverse transport accelerates wound healing via enhanced angiogenesis and immunomodulation

doi: 10.1302/2046-3758.114.BJR-2021-0364.R1

Figure Lengend Snippet: Tibial cortex transverse transport (TTT) technique enhanced small vessels in the newly formed skins. a) Wound sections were double-labelled for CD31 (red) and α-SMA (green). Nuclei were stained with 4',6-Diamidino-2-Phenylindole (DAPI, blue). Large vessels were mainly located near the edge of healthy skin, and smaller vessels were seen inside the newly formed dermis. b) Semi-quantitative measurement of immunostaining showed that the amount of blood vessels in the TTT group was significantly higher than that of the other groups (TTT vs Fixator, p < 0.001, TTT vs Sham, p < 0.001; Tukey’s multiple comparison test). Graphics were generated by Leica Application Suite. Data were measured as means (standard deviations), ***p < 0.001, n = 6.

Article Snippet: The following antibodies were used: rabbit anti-rat anti-Collagen I antibody (Col I, Abcam, UK; ab34710, 1:100); rabbit anti-rat Col III (Col III, Abcam; ab7778, 1:100); goat anti-rat CD31 (R & D Systems, USA; 10 ug/ml, AF3628); mouse anti-α-SMA (α-SMA, Abcam; ab7817, 1:100); rabbit anti-rat Anti-inducible nitric oxide synthase antibody (iNOS, Abcam; ab15323, 1:150); and rabbit anti-rat CD206 (CD206, Abcam; ab64693, 1:100).

Techniques: Staining, Immunostaining, Comparison, Generated

Journal: Cancers

Article Title: Anti-Tumor Efficacy of In Situ Vaccination Using Bacterial Outer Membrane Vesicles.

doi: 10.3390/cancers15133328

Figure Lengend Snippet: Figure 3. Analysis of Tumor-Infiltrating Lymphocytes (TILs) and cell killing by flow cytometry and immunofluorescence—24 h after one treatment with PBS or 10 µg of OMV∆60, tumors were collected and analyzed by flow cytometry and IF. The graphs in the figure reports the flow cytometry analysis of the frequency of single live cells in tumors treated with either PBS (n = 4) or with OMVs∆60 (n = 4), two plots per immunization group were also represented in the figure. Statistical analysis was performed using Student’s t test (two-tailed). * p ≤0.05, ** p ≤0.01. Error bars: mean ± SD. (A) Frequency of live cells analyzed by FACS (left) and tumor cryosections stained for apoptotic activation marker (right). Blue = Nuclei (DAPI), Green = Casp3. (B) Frequency of CD3-/NK1.1+ cells analyzed by FACS (left) and tumor cryosections stained for Natural Killer cell marker (right). Red = mouse blood vessels (CD31), Green = NKp46. (C) Frequency of CD3-/MHCII+/CD103+ cells analyzed by FACS (left) and tumor cryosection stained for activated antigen-presenting cells marker (right). Red = mouse blood vessels; Green = DC 33D1. Immunofluorescence: 20× magnification with scale bar 100 µm.

Article Snippet: CT26 tumors were stained with primary antibodies for rabbit anti-mouse Caspase 3 (Invitrogen, Waltham, MA, USA), rat anti-mouse NKp46 (Biolegend, San Diego, CA, USA), rat anti-mouse Dendritic Cell marker 33D1 (Biolegend, San Diego, CA, USA), and goat anti-mouse CD31 (R&D System, Minneapolis, MN, USA).

Techniques: Cytometry, Two Tailed Test, Staining, Activation Assay, Marker